Addgene

Showing: 1621 - 1640 of 28359 results

Results per page:

Sort results by:

pEHlyA5

Plasmid

#168298

Purpose

Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

Depositor

Luis Ángel Fernández

Article

Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

Insert

N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA

Use

Tags

E-tag, HA-tag, His-tag, Prescission site, and VHH…

Expression

Bacterial

Mutation

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-mini-Tn7T-Gm-lacZ

Plasmid

#63120

Purpose

mini-Tn7 lacZ transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Article

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
hGFAP-Cre

Plasmid

#40591

Depositor

Albee Messing

Article

Zhuo et al Genesis. 2001 Oct;31(2):85-94.

Insert

Cre recombinase (GFAP Mouse, Phage, Human)

Use

Cre/Lox and Mouse Targeting

Tags

MP-1 fragment (mouse protamine 1 intron and polya…

Expression

Bacterial and Mammalian

Mutation

Construct contains a nuclear-targeted Cre recombi…

Promoter

hGFAP promoter

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-GST-AGO2

Plasmid

#24317

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO2 (AGO2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.402

Plasmid

#187202

Purpose

Expresses retron Eco1 RT

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco1 Reverse Transcriptase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

mphR

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAD1

Plasmid

#47697

Purpose

General cloning vector for use in E. coli or D. radiodurans

Depositor

Mary Lidstrom

Article

Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

repU

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD-NTwinStrep_Sm

Plasmid

#45937

Purpose

use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-45b(+)-Tn5

Plasmid

#112112

Purpose

Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

Depositor

Frank Pugh

Article

Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

Insert

Tn5

Use

Tags

His6

Expression

Bacterial

Mutation

E54K E110K P242A L372P

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC

Plasmid

#26098

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Tags

Three consecutive Taq promoters, 2 active

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTG-mTDG

Plasmid

#52266

Purpose

Bacterial expression of mouse TDG with C-terminal GST-tag

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138129

Purpose

Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-rabbit-IgG(Fc)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Use

Tags

Expression

Bacterial

Mutation

Promoter

pAra and pTet

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS2a-RFP

Plasmid

#35328

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pExp-Sumo

Plasmid

#112574

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Use

Tags

8His and Strep tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ136

Plasmid

#162665

Purpose

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

Depositor

Gregg Beckham

Article

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Insert

MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)

Use

Tags

His

Expression

Bacterial

Mutation

codon optimized for expression in E. coli K12

Promoter

T7/lac

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge