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Plasmid#61602Purposebacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2DepositorInsertmTurqouise and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCE35
Plasmid#174409PurposeC. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFW1000
Plasmid#224210PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAJM.011
Plasmid#108529PurposeTetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.DepositorInsertTetR + PTet*-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with TEV cleavageExpressionBacterialMutationPromoterT7-lacOAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/His-miRFP670
Plasmid#79984PurposeMonomeric near-infrared fluorescent protein miRFP670 in bacterial plasmidDepositorInsertmiRFP670
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
proISG15 (2-165)
Plasmid#110762PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-FnCpf1
Plasmid#129554PurposeContains codon-optimized FnCpf1 and gRNA for streptomycesDepositorInsertFnCpf1
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPROEX-TwStrp-FTO
Plasmid#190265PurposeExpresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.DepositorInsertFTO (FTO Human)
UseTags6xHis and Twin-StreptagExpressionBacterialMutationPromoterTrcAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSJ340
Plasmid#27125DepositorInsertColicin Ia + Ia immunity protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAM552
Plasmid#154131PurposeExpresses wt rRNAs operon in bacterial cellsDepositorInsertwt bacterial rRNAs operon under control of pL promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-hPKM1
Plasmid#44241DepositorInsertPyruvate Kinase M1 (PKM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAT03-Apr
Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSm-Nuclease
Plasmid#132431PurposeExpresses Serratia marcescens nuclease in E. coli using T7 promoterDepositorInsertSerratia marcescens nuclease (codon optimized)
UseTagsNoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
BirA His-tag pLEMO
Plasmid#119817PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTags6-Histidine-tagExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD11b promoter in pGEM3zf(-)
Plasmid#26168DepositorInsertCD11b promoter (ITGAM Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
mOrange-pBAD
Plasmid#54751PurposeLocalization: Protein Expression Vector , Excitation: 548, Emission: 562DepositorTypeEmpty backboneUseTags6xHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only