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27,067 results

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pNR164

Plasmid

#79545

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR230

Plasmid

#79546

Purpose

BxbI attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR186

Plasmid

#79552

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR187

Plasmid

#79553

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR291

Plasmid

#79555

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

GFPmut3b
mTagBFP
mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR239

Plasmid

#79576

Purpose

A118 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR276

Plasmid

#79577

Purpose

TP901 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR279

Plasmid

#79578

Purpose

BxbI attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR280

Plasmid

#79579

Purpose

A118 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR287

Plasmid

#79580

Purpose

TP901 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
JMJD1CA-c089

Plasmid

#79644

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JMJD1C (JMJD1C Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
GALTA-c001

Plasmid

#79645

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GALT (GALT Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR165

Plasmid

#79646

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-SF3B2 R508K aa 401-550 fragment

Plasmid

#79674

Purpose

bacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

splicing factor 3b subunit 2 (SF3B2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 451-550 with R508K

Promoter

tac

Availability

Academic Institutions and Nonprofits only
MK14_HUMAN_D0

Plasmid

#79680

Purpose

This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK14

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
VRK3_HUMAN_D0

Plasmid

#79681

Purpose

This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK3 (VRK3 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
GAK_HUMAN_D0

Plasmid

#79682

Purpose

This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

GAK (GAK Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only