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Plasmid#70757Purposebacterial expression of human APE1DepositorInsertAPEX1 (APEX1 Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-H6_Streptavidin_sfGFP
Plasmid#124296PurposeHis6-tagged Streptavidin GFP fusion proteinDepositorInsertHis6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
UseTagsHis6 and T7 tagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAKN2_J23119-sgRNA
Plasmid#108552PurposeBacterial sgRNA expression vectorDepositorInsert2x Streptococcus pyogenes sgRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-BAIAP2 wt
Plasmid#31675DepositorInsertBAI1-associated protein 2 (BAIAP2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX-6xHis
Plasmid#145029PurposepET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tagDepositorArticleInsertRTX
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJG103
Plasmid#158533PurposewrmScarlet11 x3 tandem repeatsDepositorInsertwrmScarlet11 x3 tandem repeats
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pProEx RhoA(G14C) CL
Plasmid#224271PurposeProtein purification of RhoA (G14C)DepositorInsertRhoA (RHOA Human)
UseTagsExpressionBacterialMutationG14CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rheb(R15C) CL
Plasmid#224266PurposeProtein purification of Rheb (R15C)DepositorInsertRheb (RHEB Human)
UseTagsExpressionBacterialMutationR15CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMG-GRIS
Plasmid#225134PurposeFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-EF1a-TetOn3G
Plasmid#104543PurposePiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoterDepositorInsertTet-On 3G
UseTagsExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-40)
Plasmid#71876PurposeExpresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO1
Plasmid#53138PurposeE.coli expression of untagged mature human SUMO1 (amino acids 1-97)DepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-97)PromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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mTurquoise2-pBAD
Plasmid#54844PurposeLocalization: Protein Expression Vector , Excitation: 434, Emission: 474DepositorTypeEmpty backboneUseTags6x His, T7 tag, and TEV siteExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only