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Showing: 101 - 120 of 29412 results

  2. pET28a_PTK2b

    Plasmid
    #224237
    Purpose
    Expression of human PTK2b Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b (PTK2B Human)
    Use
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promotor
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224237-plasmid-map-sequence-id-458420

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  3. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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  5. pMSP1D1

    Plasmid
    #20061
    Purpose
    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477
    Insert
    MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-term…
    Promoter
    Available since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    20061-plasmid-map-sequence-id-354445

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  7. SuperNova/pRSETB

    Plasmid
    #53234
    Purpose
    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    Depositor
    Takeharu Nagai
    Article
    Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0
    Insert
    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    Use
    Tags
    6xHis, T7 tag, and Xpress tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53234-plasmid-map-sequence-id-236010

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  11. BirA in pET28a (w400-2)

    Plasmid
    #26624
    Depositor
    Eric Campeau
    Insert
    BirA
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26624-plasmid-map-sequence-id-228306

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  12. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

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  13. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid
    #29659
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis, SUMO, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29659-plasmid-map-sequence-id-354447

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  14. pComb3XSS

    Plasmid
    #63890
    Purpose
    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    6x His, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63890-plasmid-map-sequence-id-449948

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  15. pDG02583

    Plasmid
    #104129
    Purpose
    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    NEDD8-specific protease 1 (NEDP1)
    Use
    Tags
    14x Histidine tag, Maltose binding protein, and S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104129-plasmid-map-sequence-id-313426

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  16. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid
    #218190
    Purpose
    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Insert
    nucA
    Use
    Tags
    His and NSP4 signal secretion peptide
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218190-plasmid-map-sequence-id-467313

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  20. pVP141

    Plasmid
    #222079
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al bioRxiv 2024.08.04.606528
    Insert
    dCas9-PmCDA1, sfGFP and sacB
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222079-plasmid-map-sequence-id-438349

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Showing: 101 - 120 of 29412 results