Skip to main content
Addgene
Showing: 11581 - 11600 of 28577 results

  1. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid
    #133085
    Purpose
    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.
    Depositor
    Hana El-Samad
    Article
    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2
    Insert
    Crz1*-CLASP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pADH1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    133085-plasmid-map-sequence-id-268911

    Enlarge

  3. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101851-plasmid-map-sequence-id-200251

    Enlarge

  4. FBL

    Plasmid
    #25495
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FBL (FBL Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pDB026g

    Plasmid
    #109263
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codon at position 29, 83, 177, and 184 of g…
    Promoter
    Ppsp and PproK
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109263-plasmid-map-sequence-id-210480

    Enlarge

  6. LARS_pNIC-Bio3

    Plasmid
    #153052
    Purpose
    Expression construct for producing Avi tagged Leucyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    LARS (LARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Not full length protein
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153052-plasmid-map-sequence-id-304468

    Enlarge

  8. pGBW-m4046854

    Plasmid
    #145759
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145759-plasmid-map-sequence-id-297847

    Enlarge

  9. DsRed +9e

    Plasmid
    #191766
    Purpose
    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    DsRed
    Use
    Tags
    N terminal fusion of positively charged polypetod…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191766-plasmid-map-sequence-id-383844

    Enlarge

  10. FnR9-10

    Plasmid
    #182567
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182567-plasmid-map-sequence-id-357181

    Enlarge

  20. HGXPRT

    Plasmid
    #64201
    Purpose
    Expression of Plasmodium falciparum HGXPRT
    Depositor
    Vern Schramm
    Article
    Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi
    Insert
    Hypoxanthine guanine xanthine phosphoribosyltransferase
    Use
    Tags
    Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 11581 - 11600 of 28577 results