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pMP190

Plasmid

#185922

Purpose

Broad host range IncQ promoter probe using lacZ

Depositor

Herman Spaink

Article

Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi

Type

Empty backbone

Use

Incq

Tags

LacZ

Expression

Bacterial

Mutation

Promoter

none

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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BPK2013-AkCas12b

Plasmid

#121950

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Use

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT3-2 (C935S-Q939E)-intein-CBD

Plasmid

#73302

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Use

Tags

intein

Expression

Bacterial

Mutation

C935S-Q939E

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
PBRM1

Plasmid

#39027

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

polybromo 1 [isoform a] (PBRM1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pET22b-HA-CPDSalI

Plasmid

#38253

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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enz.079-GCN5

Plasmid

#154046

Purpose

Expresses GCN5 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone acetyltransferase GCN5 (GCN5 Budding Yeast)

Use

Tags

His, MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_CLTC_Clathrin

Plasmid

#109997

Purpose

Protein expression and purification of CLTC_Clathrin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CLTC_Clathrin (CLTC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-SCP2

Plasmid

#31336

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

sterol carrier protein 2 (SCP2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pYTK009

Plasmid

#65116

Purpose

Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pTDH3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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M64 pGEX4T-1-TEV-human beta cat domain N(1-137)

Plasmid

#17205

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino-terminal residues 1-137

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMMRSF17

Plasmid

#20178

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

C-CrkII nSH3 (CRK Human)

Use

Tags

NpuNdelC16 and Smt3

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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cp193g7N

Plasmid

#25982

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Jun 2. ():.

Insert

cp193g7N

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only
pSB1K3_pBen-sfGFP_J23101-BenR

Plasmid

#128125

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP and BenR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen and J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pet 3d alpha 1 beta 2 CP

Plasmid

#13450

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
PERK 5: PERK.K618R.pGEX4T-1

Plasmid

#21818

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) K618R (Eif2ak3 Mouse)

Use

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114. Kinase …

Promoter

Available since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGMCS-0X-P38-PanBmGFP

Plasmid

#27127

Depositor

Sabine Ehrt

Article

Gandotra et al PLoS Pathog. 2010 Aug 12;6(8):e1001040.

Insert

PanB

Use

Tags

Linker (SGSGSG), GFPm2+

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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5FAI (EMG1)

Plasmid

#79519

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

EMG1 (EMG1 Human)

Use

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO

Available since

June 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_crtEBI_19

Plasmid

#64045

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pDCyR6

Plasmid

#172558

Purpose

Inducible gene expression, pCDF origin, CymRAM repressor, PCymRC promoter, mRFP, Streptomycin Resistance Marker.

Depositor

Christopher Reisch

Insert

CymRAM-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PCymRC

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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