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Plasmid#1127DepositorInsertNM (SUP35 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSelect-2
Plasmid#190242PurposeEncodes lacI-targeting gRNA for positive selection of dCas9 activity.DepositorInsertLacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D
Plasmid#159569PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)
UseTagsHis6-MBP-tev-AviExpressionBacterialMutationG12DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.8
Plasmid#133521PurposesfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NOR gate-V2.0
Plasmid#44452DepositorInsertNOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3σ
Plasmid#111881PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3σ fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
JBEI-3716
Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pXRA2
Plasmid#63144PurposeADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS
Plasmid#98566PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein
UseTagsExpressionBacterialMutationIn a synthetic operon downstream of UBP1 and Remo…PromoterSame as UBP1 (operon) and Tet promoter (aTc induc…Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHIP1-SH2
Plasmid#46492DepositorInsertSHIP1 (INPP5D Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-118PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG03'
Plasmid#162578PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_T
Plasmid#74068PurposepAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecRDepositorInsertsgRNA pck (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W
Plasmid#109416PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR43W repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK080
Plasmid#171352PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTcyc1 YJR048W terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK079
Plasmid#171351PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertToat1 Pc18g03600 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK055
Plasmid#171327PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertrTA2S-M2 (TetR-3xVP16) transcriptional activator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK052
Plasmid#171324PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertFirefly luciferase reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (L135V)
Plasmid#31154DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationLeucine 135 changed to ValinePromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only