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pF708-pET28a-Hs-delta38-METTL20-NHis wt

Plasmid

#85109

Purpose

expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta38) METTL20 (METTL20 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i3-TEV-Strep

Plasmid

#174501

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i3

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC017.109

Plasmid

#171145

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSG 34-11

Plasmid

#63869

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCoofy37

Plasmid

#43998

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pJOE6227.1

Plasmid

#135078

Purpose

An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

up- and downstream regions of upp (upp Pseudomonas putida)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEN41A-T10M

Plasmid

#27365

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

tetR(sB)

Use

Gateway donor vector

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

June 21, 2011

Availability

Academic Institutions and Nonprofits only

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pSopB2xHA

Plasmid

#112513

Purpose

PsopB driven sopB-2xHA and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB-2xHA/sigE operon

Use

Tags

2xHA

Expression

Bacterial

Mutation

SopB C-terminal 2xHA

Promoter

PsopB

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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ACOT12BA

Plasmid

#42395

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ACOT12BA (ACOT12 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pAT695

Plasmid

#68910

Purpose

NanoLuc expressed constiutively from PBT1311 and RC500 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT587

Plasmid

#68906

Purpose

NanoLuc expressed constiutively from PBT1311 and GH022 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Use

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEW65: pQE-81L H3(K79M)

Plasmid

#124100

Purpose

H3K79M expression plasmid for norleucine supplementation

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Histone H3 (LOC108704296 Frog)

Use

Tags

Expression

Bacterial

Mutation

K79M

Promoter

T5

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPotef-LN22*-3xGNLS-cbx

Plasmid

#86780

Purpose

Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

Depositor

Michael Feldbrügge

Article

Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

Insert

LN*

Use

Ustilago maydis expression

Tags

Gfp

Expression

Bacterial

Mutation

Promoter

Potef

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CYP11A1 (3N9Y)

Plasmid

#28119

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-Gluc-EL222-his

Plasmid

#172097

Purpose

Expresses the Gluc-EL222 fusion protein in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - EL222

Use

Tags

Expression

Bacterial

Mutation

Gluc mutations - M43L, M110L

Promoter

T7

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pBMTBX-3

Plasmid

#26074

Depositor

Ryan Gill

Article

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2010

Availability

Academic Institutions and Nonprofits only

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