Addgene

Showing: 10621 - 10640 of 28539 results

Results per page:

Sort results by:

pJBL7032

Plasmid

#133873

Purpose

AtzR expression plasmid

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

Insert

AtzR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CARD14-G117S

Plasmid

#69920

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G117S

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pKM263-EcPPXc

Plasmid

#38328

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Use

Tags

6xHIS, GST, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT106b

Plasmid

#31392

Depositor

Christopher Voigt

Article

Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

Insert

red light inverter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD280

Plasmid

#125071

Purpose

Expression of SO_4387 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4387 (SO_4387 )
lacI (lacI )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD279

Plasmid

#125099

Purpose

Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4388(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

Plasmid

#176535

Purpose

Non-protein coding locus components from CRISPR-associated IscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM42

Plasmid

#113942

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1a

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRGECO1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-auxilin(547-910)

Plasmid

#62572

Purpose

Codes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruption

Depositor

Ernst Ungewickell

Article

Holstein et al J Cell Biol. 1996 Nov;135(4):925-37.

Insert

Auxilin

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only
pTJV1Sc-rpoB

Plasmid

#166978

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

Ec86 Retron (ECD_00831 )
lambda bet

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101 and VanCC

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE-miR21-SunTag-TREAT

Plasmid

#196932

Purpose

SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(I) pXYL (J) P3 (SBE108)

Plasmid

#194991

Purpose

promoter of the gene xylose reductase from R. toruloides for a position P3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pXYL (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXTL-T7max-HisGFPHiBiT

Plasmid

#188316

Purpose

Expression of GFP-HiBiT via T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

GFP-HiBiT fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXTL-T7max-HiBiTGFPHis

Plasmid

#188317

Purpose

Expression of HiBiT-GFP via T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

HiBiT-GFP fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HS-DN-Plk1

Plasmid

#105949

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk1 (PLK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Dominant negative K82 R

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge