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Plasmid#103941PurposeProtein expression and purification of RIL BGLII PDZ domainDepositorInsertPDLIM4 (PDLIM4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos3-dnRac1
Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCML 961
Plasmid#98848PurposepTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).DepositorInserts5' UTR + first 100 nucleotides of coding sequence of the aidB gene
csrA
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLY70
Plasmid#130948PurposeA CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).DepositorInsertsdxcas9 (3.7)
tetR
rhaS
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9 for dCas9; Sy…PromoterPcon, PpspA-LEA3B3, PrhaB, and PtetAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP-MGapt
Plasmid#67734PurposeOR2-OR1-Pr promoter expressing deGFP-MG aptamer fusionDepositorInsertUTR1-deGFP-MGapt-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB675
Plasmid#176578PurposeExpressing three of the five genes of the n-butanol pathway.DepositorInsertphaJ, ter, adhE2
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-2ndVal mGFPmut2
Plasmid#104033PurposePlasmid encoding 2ndVal mGFPmut2DepositorInsert2ndVal mGFPmut2
UseLow copyTagsExpressionBacterialMutationmGFPmut2 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
C2-82 pComb3xss anti-cFos nanobody
Plasmid#145838PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C2-82 (FOS L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HS-DN-Ect2
Plasmid#105956Purposeheat shock inducible transgenesisDepositorInsertEct2 (ECT2 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EPS15_EH
Plasmid#178475PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertEPS15_EH (EPS15 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 C450A
Plasmid#81031PurposeMammalian expression of the dark state of LOV2DepositorInsertLOV2
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450APromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP-HSP90 (3H80)
Plasmid#25273PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLmjF33.0312:M1-K213
UseTagsHisExpressionBacterialMutationContains amino acids M1-K213PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGEX6P1-DEST-HA
Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -