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Plasmid#26640DepositorInsertAHU
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
PGEX-6P1-hNF2-AR mutant
Plasmid#107149Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTagsGSTExpressionBacterialMutationA585W-R588KPromoterPtacAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-His6
Plasmid#51532PurposeExpress His6-tagged hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-GFP-RGG
Plasmid#124949PurposeExpression of MBP-RGG-GFP-RGGDepositorInsertMBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsEGFP, His, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5-2300
Plasmid#171788PurposeEncodes sgRNA target 2300 and Gentamicin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5
Plasmid#64985PurposeExpresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLacIq-Wasabi
Plasmid#86112PurposemWasabi reporter gene driven by LacIq promoterDepositorInsertmWasabi
UseTags6xHisExpressionBacterialMutationPromoterpLacIqAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoofy41
Plasmid#55184PurposeInsect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTagsHis10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pOP5GT
Plasmid#112611PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GST and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Col1A-5'probe
Plasmid#20732DepositorInsertCol1A-5'probe (Col1a1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRL542
Plasmid#70282PurposeColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-EmGFP-15b
Plasmid#138280PurposeExpress tTALE-EmGFPDepositorInsert7-mer tTALE-EmGFP
UseTALENTags6x His-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -