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  2. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

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  4. pET22-6His-SUMO-Nsp10

    Plasmid
    #154960
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154960-plasmid-map-sequence-id-306350

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  8. pTorPE-B-GECO1

    Plasmid
    #32469
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    B-GECO1.0
    Use
    Lab constructed
    Tags
    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…
    Expression
    Bacterial
    Mutation
    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…
    Promoter
    Available since
    Oct. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    32469-plasmid-map-sequence-id-37242

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  11. pGBW-m4046942

    Plasmid
    #145757
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145757-plasmid-map-sequence-id-292503

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  14. eGFP-Silicatein-pET28

    Plasmid
    #198012
    Purpose
    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification
    Depositor
    Bryan Berger
    Article
    Vigil et al Materials Advances 2, 2023
    Insert
    eGFP-silicatein
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198012-plasmid-map-sequence-id-388331

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  16. pSPYngK+-hp

    Plasmid
    #48121
    Purpose
    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    codon optimized signal peptide of YngK
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48121-plasmid-map-sequence-id-69947

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Showing: 10061 - 10080 of 28540 results