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pEB1-coCeruleanME

Plasmid

#104016

Purpose

Plasmid encoding coCeruleanME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coCeruleanME

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as Cerulean ME (codon optim…

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBbA2a-RFP

Plasmid

#35325

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRa

Plasmid

#134990

Purpose

dCas9-mediated activation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
pET14b PHAS-I F113A

Plasmid

#15680

Depositor

John Lawrence

Article

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I F113A (Eif4ebp1 Rat)

Use

Tags

6xHis

Expression

Bacterial

Mutation

F113A mutation.

Promoter

Available since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
LB1366 (Bcl-2 promoter with MHS1234)

Plasmid

#15388

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
NELFE-mOrange pHis-//

Plasmid

#53303

Purpose

Expresses His-tagged NELF-E-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

NELF-E protein (Nelf-E Fly)

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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HS-Utrophin-GFP

Plasmid

#105952

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Utrophin (Utrn Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-Clover

Plasmid

#103991

Purpose

Plasmid encoding Clover

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Clover

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD-10xHis)

Plasmid

#176192

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG7(gfcB)

Plasmid

#176196

Purpose

gfcB protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcB

Use

Tags

6xHis-TeV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG26(gfcD)

Plasmid

#176198

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcB-His-CD)

Plasmid

#176194

Purpose

expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Use

Tags

GfcB-6xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD)

Plasmid

#176199

Purpose

Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

pelC leader-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD-10xHis)

Plasmid

#176200

Purpose

Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

pelC leader-10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcBCD)

Plasmid

#176193

Purpose

expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-RevA-ss

Plasmid

#137064

Purpose

E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAF07416.1 (revA Borrelia burgdorferi B31)

Use

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

lacks the RevA signal peptide and 5 residues (CKA…

Promoter

T7lac

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTrc99A-Rat FABP1

Plasmid

#13577

Depositor

Martin Scanlon

Article

Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

Insert

Rat liver fatty acid binding protein (Fabp1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only
Sup35 ScNM A230C His7

Plasmid

#15598

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Budding Yeast)

Use

Tags

7xHis

Expression

Bacterial

Mutation

A230C. Alanine 230 mutated to cysteine.

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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