GST-GAR
(Plasmid #34697)

• Depositing Lab: Steven Clarke
• Publication: Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-82.

Backbone

• Vector backbone: pGEX
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fibrillarin
• Species: H. sapiens (human)
• Insert Size (bp): 444
• Mutation: Residues 1-145 of human fibrillarin with K2E and A145V substitutions preceded by a linker region of SDLVPRGS. This region contains 15 arginine residues in a glycine-rich region.
• Entrez Gene: FBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: pGEX 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Addgene quality control sequence only verified 145 amino acids. The depositor notes that this should not be a problem for scientists using this construct as a methyl-acceptor.