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pET28a-cMSP26

Plasmid

#199769

Purpose

Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP26

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Promoter

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH4-6

Plasmid

#199766

Purpose

Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH46

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSB4A3-PBAD-phzH

Plasmid

#165618

Purpose

Arabinose-inducible expression of phzH for conversion of phenazine-1-carboxylic acid to phanzine-1-carboxamide

Depositor

Martin Buck

Insert

phzH (phzH Pseudomonas aeruginosa)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV2-2/2

Plasmid

#91506

Purpose

Protein expression and purification of human SH3 domain construct VAV2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV2-2/2 (VAV2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOXO1-1/2

Plasmid

#91499

Purpose

Protein expression and purification of human SH3 domain construct NOXO1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXO1-1/2 (NOXO1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-NEMOD311N

Plasmid

#11968

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOD311N (IKBKG Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Asp311 to Asn

Promoter

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pAT937

Plasmid

#68936

Purpose

Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int12

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJF131B

Plasmid

#113326

Purpose

pTet-W108 scRNA.b2, pTet-RR2-sgRNA

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2 and sgRNA_RR2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-M.accABCD

Plasmid

#66865

Purpose

Encodes accA, accB, accC, accD in monocistronic configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSkunk3-BLA

Plasmid

#61531

Purpose

Phagemid expressing streptomycin/spectinomycin resistance gene

Depositor

Marc Ostermeier

Article

Firnberg et al PLoS One. 2012;7(12):e52031. doi: 10.137

Insert

aadA

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacI

Available since

Dec. 12, 2014

Availability

Academic Institutions and Nonprofits only

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E17TAG GFP zeo resistance

Plasmid

#69118

Purpose

For E17TAG GFP reporter expression in non-zeocin resistant strains

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Use

Tags

His

Expression

Bacterial

Mutation

E17 to TAG

Promoter

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only
pC236

Plasmid

#65951

Purpose

Medium Repressor plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

CinI

Use

Tags

LAA tagged

Expression

Bacterial

Mutation

Promoter

Prhl/lac-m

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pRS513

Plasmid

#17283

Depositor

Nancy Craig

Article

Skelding et al EMBO J. 2002 Jul 1. 21(13):3494-504.

Insert

TnsB (tnsB E. coli transposon Tn7)

Use

Tags

Chitin-binding domain-intein

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pGEX Lyn-SH2

Plasmid

#46452

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lyn (LYN Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 106-213

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Use

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet1_CoiC_CoiSA

Plasmid

#208762

Purpose

Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiC
CoiSA

Use

Tags

Expression

Bacterial

Mutation

single nucleotide insertion (C70) at RBS of CoiC

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRAF-MutH

Plasmid

#86609

Purpose

E. coli MutH expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutH

Use

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBT-4

Plasmid

#22831

Depositor

Ryan Gill

Article

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pMS31: pTwinStrep-SUMO-PmcTnsAB

Plasmid

#168164

Purpose

PmcTnsAB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST TnsAB

Use

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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PIM1

Plasmid

#39132

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PIM1 (PIM1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only