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pCellFree_G06_ZIKVC

Plasmid

#145349

Purpose

Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk1a1

Plasmid

#15743

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a1 (MAPK8 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Use

Tags

His6-hPDI

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B28-Leum

Plasmid

#44327

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B28-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B28-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGST-GstZ1

Plasmid

#163090

Purpose

Expressing mouse Gstz1 in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

GstZ1
GstZ1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS2B

Plasmid

#145344

Purpose

Cell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS4B

Plasmid

#145347

Purpose

Cell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI-FLAG

Plasmid

#186560

Purpose

Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTKIP-cat

Plasmid

#41065

Depositor

Edward Cox, Thomas Kuhlman

Article

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAT588

Plasmid

#68907

Purpose

NanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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ataxin3:1-431_Q79_BV

Plasmid

#209733

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

6xHis-SBP-TEV

Expression

Bacterial

Mutation

Promoter

Polyhedrin

Available since

Nov. 13, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CBLB

Plasmid

#39056

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CBLB (CBLB Human)

Use

Tags

TEV-His6-Flag

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pC165

Plasmid

#65949

Purpose

P1 activator plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

RhlI (rhlI )

Use

Tags

LAA tagged

Expression

Bacterial

Mutation

Promoter

pLLac*

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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LB1376 (Bcl-2 P1 promoter with MHS1234)

Plasmid

#15387

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 P1 promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72094

Plasmid

#40778

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pHD_v2

Plasmid

#32183

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer HD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pNG_v2

Plasmid

#32181

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer NG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_E299A

Plasmid

#91855

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) E299A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

E299A

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRF-IcaR

Plasmid

#49362

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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