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Showing: 9281 - 9300 of 28534 results

  2. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  3. SCLYA

    Plasmid
    #42358
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SCLYA (SCLY Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pOPS0359

    Plasmid
    #115508
    Purpose
    Reporter Plasmid. Taurine-inducible expression of GFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Taurine-inducible promoter of S. meliloti Promote…
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115508-plasmid-map-sequence-id-224277

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  14. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid
    #101718
    Purpose
    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101718-plasmid-map-sequence-id-196776

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  15. pOGG012

    Plasmid
    #113986
    Purpose
    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Partition genes (parABCDE)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113986-plasmid-map-sequence-id-223365

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  20. pOGG004

    Plasmid
    #113979
    Purpose
    Vector construction module. Level 1 golden gate cloning site module with LacZ reporter
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Level 1 golden gate cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113979-plasmid-map-sequence-id-223412

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Showing: 9281 - 9300 of 28534 results