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Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
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pIT5_TL
Plasmid#173768PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryabb
Plasmid#47665PurposeRecombinant protein productionDepositorInsertAlpha B-crystallin (cryabb Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-cat
Plasmid#71321PurposeDonor plasmid - chloramphenicol resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-Luc
Plasmid#135627PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)DepositorInsertNAD-Luc sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOP2S
Plasmid#112597PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusionsDepositorTypeEmpty backboneUseTagsStrep tagExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-AcrB/AcrD/AcrFa, ABO_0964
Plasmid#86206PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
P74-26 Tail Tube Protein (gp93) in pSMT3
Plasmid#191261PurposeExpresses P74-26 gp93 with a His-SUMO tag in bacterial cellsDepositorInsertP74-26 gp93
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AND-gate-V2.0
Plasmid#44449DepositorInsertAND-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dHer1cys
Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
UseTags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SM
Plasmid#136578PurposeDox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMycDepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWarf(+)
Plasmid#34562DepositorInsertHRV 3C-GPA-GFP-8HIS
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
CHD1 (CHD1A-c107)
Plasmid#110253PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCHD1 (CHD1 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-P1Egfp
Plasmid#90148Purposecontains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aE1P1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only