-
-
pTDpelB-CTwinStrep
Plasmid#45941Purposeuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneUseTagsPelB export signal and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorInsertHOXC9 (Hoxc9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHN873
Plasmid#178829PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsMBP, His8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-TTGZFP-FokI-DD
Plasmid#18755DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD
Plasmid#73300PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationN1043S-Q1047E-S1079N-E1083QPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos3.2-A69T
Plasmid#98571Purposephotoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmEos3.2-A69T
UseTags7xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLIParaF.Ec-250n
Plasmid#18855DepositorInsertarabinose FRET sensor
UseTagsCFP and VenusExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAM5087
Plasmid#85123PurposePtrc-YFP subcloned from pAM5080 into NSII-Km vector.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Ets1-ETS
Plasmid#85735PurposeExpresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440DepositorInsertDNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Human, Mouse)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFCiR2
Plasmid#167512PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCinRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D
Plasmid#159543PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationG13DPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-APEX2-F
Plasmid#160083PurposeBacterial expression of C-terminally FLAG-tagged APEX2 peroxidaseDepositorInsertAPEX2 peroxidase
UseTagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorInsertJNK1a1 (MAPK8 Human)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-161
Plasmid#118541PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-566
Plasmid#104195PurposeExpresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusionDepositorInserthuman androgen receptor aa 1-566 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
NTERY01
Plasmid#184119PurposeNanopore-addressable protein reporter for expression in bacterial cells.DepositorInsertNTERY01
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_trigger
Plasmid#132745PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNADepositorInsertToeRepG2_N02_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only
