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Plasmid#28061DepositorInsertEZH2 (EZH2 Human)
UseTagsGSTExpressionBacterialMutationT487APromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIB163
Plasmid#90186PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoterDepositorTypeEmpty backboneUseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG126
Plasmid#105836PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 126PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pvdA WT
Plasmid#87098Purposeexpresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosaDepositorInsertpvdA WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI
Plasmid#115213PurposeExpresses RanCas13b from a SUMO-tagged bacterial expression constructDepositorInsertRanCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)8k-sfGFP
Plasmid#110140PurposeIPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 originDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP-NMT
Plasmid#25200PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLmjF32.0080:P5-L421
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28_mouse-pro-IL18
Plasmid#183389PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL18 (Il18 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18-mini-Tn7T-Gm-LacZ10
Plasmid#65026PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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Chx10 3kb AP (CC#318)
Plasmid#15205DepositorInsertChx10 3kb AP reporter (Vsx2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI2_G-alpha
Plasmid#109791PurposeProtein expression and purification of GNAI2_G-alphaDepositorInsertGNAI2_G-alpha (GNAI2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AFDN_PDZ_1
Plasmid#103938PurposeProtein expression and purification of TRITHORAX PDZ domainDepositorInsertAFDN (AFDN Human)
UseTagsGSTExpressionBacterialMutationcontains AA983-1110 of AFDNPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP2774
Plasmid#160667PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p32m3C Trx-CC
Plasmid#196475PurposeProtein expression of Thiolredoxin-3C cleavage site-CarHCCDepositorInsertTrx-CC
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pf-GMPS
Plasmid#36899PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPf-GMPS (PF10_0123 Plasmodium falciparum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE-Tn5-156
Plasmid#118538PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only