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pGEX Hck-SH2

Plasmid

#46445

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Hck (HCK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 134-254

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-cpLuc

Plasmid

#135628

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc sensor

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCS4

Plasmid

#55751

Purpose

Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Use

Tags

Expression

Bacterial

Mutation

Residues 1-59 of KanR

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSG22-pBAD-TetR

Plasmid

#102451

Purpose

Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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RBM23_2xRRM_pGEX

Plasmid

#135118

Purpose

Recombinant expression of RBM23 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM23 (RBM23 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5048:Dendra

Plasmid

#167366

Purpose

C-term fluorescent fusion

Depositor

Joseph Wade, Keith Derbyshire

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5048

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP2824

Plasmid

#160671

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET mRuby2 LIC cloning vector (H6-mRuby2)

Plasmid

#86934

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

Biotin, His6, Tev Recognition Sequence, and mRuby…

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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Reb_82 aka PAS623

Plasmid

#71228

Purpose

Reb_1 with RebB(S82P, D88G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Use

Tags

none

Expression

Bacterial

Mutation

RebB(S82P, D88G), RebA (G53A)

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Reb_67 aka PAS627

Plasmid

#71232

Purpose

Reb_1 with RebA(S88A)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Use

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, S88A)

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pFTK004

Plasmid

#171276

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgpdA ANIA_08041 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDA183

Plasmid

#74247

Purpose

pSTL1-dPSTRr(2,1)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pSTL1-dPSTRr(2,1)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp2-GFPuv

Plasmid

#139083

Purpose

Low phosphate inducible promoter yibDp2 for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yibDp2-GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xAU1

Plasmid

#129572

Purpose

Empty backbone for AU1 tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Use

Tags

3xAU1 and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEB1-coVenNB

Plasmid

#104019

Purpose

Plasmid encoding coVenNB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coVenNB

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as Venus NB (codon optimize…

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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