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Plasmid#112599PurposeUsed for expression of protein in E. coli as N-terminal Sumo tag fusionsDepositorTypeEmpty backboneUseTagsSumoExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only
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pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGMCSq17-10M-0X
Plasmid#27364DepositorInserttetR(sB)
UseGateway destination vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV115
Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDF0039 DisCas7-11 only expression with CRISPR array
Plasmid#172500PurposeEncodes the DisCas7-11 protein only for bacterial expressionDepositorInsertDisCas7-11 only expression with CRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH291
Plasmid#172153PurposeReporter plasmid for pylBCD variant in pCDF backboneDepositorInsertPara sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
UseTagsExpressionBacterialMutationsfGFP(N39*, Y151*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
human rSHIP2 (18-893)
Plasmid#128568PurposeExpression of human SHIP2 in bacteriaDepositorInsertSHIP2 (INPPL1 Human)
UseTagsHis Xpress (N-terminal)ExpressionBacterialMutationSHIP2 coding sequence from amino acid 18 (A) till…PromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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PARP15-c020
Plasmid#173101PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
UseTagsmCeruleanExpressionBacterialMutationN78-S267, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2849
Plasmid#160677PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DYNLL1_Dynein-light
Plasmid#110006PurposeProtein expression and purification of DYNLL1_Dynein-lightDepositorInsertDYNLL1_Dynein-light (DYNLL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆FHA-mNeonGreen-IRESpuro2
Plasmid#183739PurposeExpresses Ki67∆FHA-mNeonGreenDepositorInsertMKI67_lacking FHA (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking FHA)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ0135
Plasmid#11412DepositorInsertRNase HII (MJ_RS00700 M. jannaschii)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only