human rSHIP2 (18-893)
(Plasmid #128568)

• Purpose: Expression of human SHIP2 in bacteria
• Depositing Lab: Christophe Erneux
• Publication: Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

Backbone

• Vector backbone: pTrc-HIS
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4400
• Total vector size (bp): 7022
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SHIP2
• Alt name: SHIP2; 51C protein; INPPL1
• Species: H. sapiens (human)
• Insert Size (bp): 2622
• Mutation: SHIP2 coding sequence from amino acid 18 (A) till amino acid 893 (K) from the EMBL sequence data base Y14385
• GenBank ID: NM_001567
• Entrez Gene: INPPL1 (a.k.a. OPSMD, SHIP2)
• Tag / Fusion Protein:
  • His Xpress (N-terminal) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pTrc His forward primer
• 3′ sequencing primer: pTrc His reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry