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pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 His10-FcRɣ
Plasmid#113021Purposefor purification of His10-FcRɣ intracellular domainDepositorInsertHis10-FcRɣ intracellular domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX PLCg1(C)-SH2
Plasmid#46469DepositorInsertPLCg1(C)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 656-766PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMS18: pHelper(PmcCAST)_PmcPSP1
Plasmid#168151PurposeInducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP577
Plasmid#98801PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
UseTagsExpressionBacterialMutationI138T, N171D, N176TPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pEB2-mCherry-L
Plasmid#104002PurposePlasmid encoding mCherry-LDepositorInsertmCherry-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE DNLA IIKEF… V…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA3
Plasmid#52263PurposeBacterial expression of human 6His-tagged SUMO3 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBLO 4.01
Plasmid#121495Purposeengineered Mu transposonDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE3c
Plasmid#17463Purposemodified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB021CH(+)
Plasmid#99209PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; ProK pylT
UseTagsExpressionBacterialMutationPromoterPT7 and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1
Plasmid#87737PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD317.8
Plasmid#181951PurposeIPTG-inducible expression of NarXDepositorUseTagsExpressionBacterialMutationPromoterNarX-Ptac; lacI-PlacIqAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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NTERY00
Plasmid#184109PurposeNanopore-addressable protein reporter for expression in bacterial cells.DepositorInsertNTERY00
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
NTERY02
Plasmid#184120PurposeNanopore-addressable protein reporter for expression in bacterial cells.DepositorInsertNTERY02
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only
