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Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
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pALACE
Plasmid#31853DepositorTypeEmpty backboneUseEscherichia coli-mycobacterium shuttle plasmidTags6xHISExpressionBacterialMutationPromoterAcetamidase operoneAvailable sinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S222TAG
Plasmid#68302PurposeN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expressionDepositorInsertMBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
HARS_pNIC-Bio3
Plasmid#153050PurposeExpression construct for producing Avi tagged histidyl tRNA synthetaseDepositorInsertHARS (HARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKm
Plasmid#170331Purposebackbone vector for clone constructionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEXtev-HMG54kd
Plasmid#26030DepositorInsert3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
UseTagsGSTExpressionBacterialMutationCatalytic domain (amino acids 426-488) Methion…PromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_4
Plasmid#103922PurposeProtein expression and purification of PTN13 PDZ4 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo1
Plasmid#157805PurposeExpression of synthetic protein composed of eGFP and BRD4 bromodomain #1DepositorInserteGFP-bromo1
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExp-Trx
Plasmid#112577PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TrxA and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-SmBiT
Plasmid#207632PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiTDepositorInsertphotocaged SpyCatcher-SmBiT
UseTags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP120
Plasmid#71732Purposeinducible expression of TRP120 in E. coliDepositorInsertTRP120
UseTags6xHIS and ThioredoxinExpressionBacterialMutationPromoterpBADAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SRSF9_2xRRM_pGEX
Plasmid#135120PurposeRecombinant expression of SRSF9 with dual-affinity tagDepositorInsertSRSF9 (SRSF9 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
lacI-Ptac-gusA-pBAV1k
Plasmid#30503DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-Rac1 Q61L
Plasmid#81137Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationQ61LPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS/KS-mId3sp
Plasmid#16045DepositorInsertId3 (Id3 Mouse)
UseTagsExpressionBacterialMutationcDNA nucleotides 365-635PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P1
Plasmid#74685PurposePhlF-P1 gateDepositorInsertPTac-RBSP1-PhlF-PPhlF-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S1
Plasmid#74691PurposeSrpR-S1 gateDepositorInsertPTac-RBSS1-SrpR-PSrpR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only