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Plasmid#14465DepositorInsertunstable gfp-mut3.1 LVA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDB24
Plasmid#91959Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneUseTags10-His and Smt3ExpressionBacterial and YeastMutationPromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28-His8-aCat(660-906)
Plasmid#203333PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-906 onlyPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-35-H-6
Plasmid#67008PurposeExpresses malE-35-H-6 in E. coliDepositorInsertmalE-35-H-6
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRF-BetI
Plasmid#49357DepositorInsertBetI repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA5-Halo
Plasmid#175326PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA5 (HSPA5 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK651
Plasmid#71702PurposeProduces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)DepositorInsertthioredoxin reductase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn
Plasmid#100947PurposeExpression of Tyrosine Ammonia Lyase from inducible T7 promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-hphMX6
Plasmid#105159PurposeYeast gene targetingDepositorInsertGBP-hphMX6
UseTagsGBPExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15
Plasmid#153523PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#15DepositorInsertSb#15
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAQ_14-3-3
Plasmid#109943PurposeProtein expression and purification of YWHAQ_14-3-3DepositorInsertYWHAQ_14-3-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-lambdaRED_NOC
Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV105
Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
UseTagsExpressionBacterial and YeastMutationPromoterGAL1 GAPDHAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII
Plasmid#209292PurposeExpresses R2Bm-Cas9(H840A) fusion in E. coliDepositorInsertR2
UseTagsHis, MBP, SpCas9(H840A), StrepII, and bdSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKEW106-MbCas12a-58069
Plasmid#115653PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only