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Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only
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pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
UseTagsHalo and His6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
UseTags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionine--tRNA ligase (metG Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-ASF1A
Plasmid#31591DepositorInsertASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X7
Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mJuniper
Plasmid#219398PurposeConstitutive expression of mJuniper in E. coliDepositorInsertmJuniper
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
VHL-pGex2TK
Plasmid#20790DepositorInsertVHL (VHL Human)
UseTagsGSTExpressionBacterialMutationVHL wt cDNA, no mutationsPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-ThrRS
Plasmid#124119PurposeBacterial expression plasmid for PURE system componentsDepositorInsertThreonyl-tRNA ligase (thrS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chAcK3RS(IPYE)
Plasmid#104069PurposeExpression plasmid encoding chAcK3RS(IPYE) and pylTDepositorInsertPlpp chAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTP1183
Plasmid#104163PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-CM
Plasmid#203588PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LysRS-His
Plasmid#124114PurposeBacterial expression plasmid for PURE system componentsDepositorInsertLysine-tRNA ligase (lysU Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLychee
Plasmid#219400PurposeConstitutive expression of mLychee in E. coliDepositorInsertmLychee
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ProRS-His
Plasmid#124117PurposeBacterial expression plasmid for PURE system componentsDepositorInsertProlyl-tRNA ligase (proS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PIA900
Plasmid#104401PurposePolycistronic vector for expression of E. coli core RNAPDepositorInsertrpoA, B, C, and Z
UseE. coli expression vectorTagsencodes a C-terminally His-tagged rpoC which can …ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only