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Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbA8k-RFP
Plasmid#35273DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
UseTagsExpressionBacterialMutationS111G/A137L/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2-TS_pET28a
Plasmid#215421PurposeExpression construct for NylCp2-TS gene, codon optimized for expression in E. coliDepositorInsertNylCp2-TS
UseTagsExpressionBacterialMutationD122G/H130Y/D36A/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript SK +/- mTRF1
Plasmid#12303DepositorInsertmTRF1 (Terf1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_G47E
Plasmid#101779PurposeExpresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationG47E mutationPromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW194b63
Plasmid#136930PurposeDirected evolution of SpCas10DepositorInsertnpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAV-A
Plasmid#65927PurposeTheophylline Riboswitch A with lacZ geneDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterT5Available sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_18
Plasmid#59953PurposeQuad split (at 61, 179, 601-SZ)DepositorInsertFour-fragment T7* RNA polymerase
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_TS
Plasmid#140667PurposeToggle switch (TS)DepositorInsertToggle switch (TS)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCYP01
Plasmid#212834PurposeExpresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
P25-pZE-SUMO-PbTTA
Plasmid#204634PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-PbTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S218TAG
Plasmid#68301PurposeN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expressionDepositorInsertMBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Myc
Plasmid#119752PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p406MET3
Plasmid#17436DepositorInsertMET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_27
Plasmid#59958PurposePTac null fragment (sigma CGG Y639A) expressionDepositorInsertnull fragment
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only