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Plasmid#49423PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterCUP1Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKEW189-MBP-TEV-AcrVA1
Plasmid#115660PurposeExpresses AcrVA1 in bacteria via T7 RNAP, for purificationDepositorInsertAcrVA1
UseTagsMBP-TEVExpressionBacterialMutationPromoterT7 RNAPAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScaf-10080.1
Plasmid#111410PurposepScaf phagemid with insert for generating DNA origami scaffold 10080 bases in lengthDepositorInsertpScaf-ACKN
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Z-VCP-10
Plasmid#166559PurposescFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPotef-cdc3CB16-NatR
Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialMutationPromoterPotefAvailable sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS12
Plasmid#192468PurposeBacterial expression of RvCAHS12DepositorInsertCAHS12
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF5
Plasmid#37219DepositorInserteIF5
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Slow-FT
Plasmid#31918DepositorInsertSlow-FT
UseTagsHisExpressionBacterialMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBEN1-SGC
Plasmid#39127PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsStrep tag and SET1ExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKD280.7
Plasmid#173172PurposeExpresses SO_4387DepositorInsertSO_4387
UseTagsExpressionBacterialMutationPromoterJ23107Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SRSF8_RRM_pGEX
Plasmid#135119PurposeRecombinant expression of SRSF8 with dual-affinity tagDepositorInsertSRSF8 (SRSF8 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-N268D
Plasmid#24861DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationAsparagine 268 mutated to AspartatePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
HS-Par3-GFP
Plasmid#105947Purposeheat shock inducible transgenesisDepositorInsertPar3
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2(plus)EGFP_H
Plasmid#146446PurposeBacterial Expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_(27G2)_FLAG
Plasmid#199616PurposeEncodes DARPin-FLAG 27G2 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27G2
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -