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Plasmid#168195Purposeexpresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.DepositorInserteatA[H134R]
UseTagsExpressionBacterialMutationH134R mutationPromoteraraBADAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQMD
Plasmid#48103PurposeDestination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion proteinDepositorTypeEmpty backboneUseTagsmyc tagExpressionBacterialMutationPromoterPQ5Available sinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDE43-MCSq17
Plasmid#27333DepositorInsertqtag17
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-RFP
Plasmid#199652PurposeR6K backbone with Golden Gate (GG) cloning sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPJ23116-MS2-rep
Plasmid#184842PurposeExpression of the rep protein of phage MS2DepositorInsertReplicase gene MS2 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.416
Plasmid#187207PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2)
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCTD
Plasmid#157871Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaCTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
APneg-VAMP1a
Plasmid#168007Purposenegative selection PACE Accessory PlasmidDepositorInsertVAMP1(T29-K89)-T7PAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9(WT)
Plasmid#101044PurposeExpress Streptococcus pyogenes Cas9DepositorInsertSpyCas9
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINEneo-3C-GFP
Plasmid#53534PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
UseTagsGFP-His8ExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-131
Plasmid#185923PurposeHimar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein geneDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.713
Plasmid#108514PurposePLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.DepositorInsertPLuxB-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT14.G
Plasmid#201002PurposepLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11
Plasmid#200991PurposepLv1-pUC18R6KT-miniTn7-Gm, GmR, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS96
Plasmid#207588PurposeGFP-TonBH20A expressionDepositorInsertGFP-TonBH20A
UseTagsExpressionBacterialMutationH20APromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS65
Plasmid#207587PurposeGFP-TonB expressionDepositorInsertGFP-TonB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CN-B
Plasmid#196477PurposeProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCNDepositorInsertB-CN-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only