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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Use

Tags

C-terminal 6xHis

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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803 pGEX2T PTEN

Plasmid

#10784

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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GST-FUS 465-526

Plasmid

#44983

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (465-526aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 465-526

Promoter

tac

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
pM

Plasmid

#64179

Purpose

harbours marker gene MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-cMyc

Plasmid

#136614

Purpose

Dox-inducible lentiviral vector expressing mouse cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

cMyc (Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-CDC27 wt

Plasmid

#31673

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog (CDC27 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-Rx3(A7)

Plasmid

#35525

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

His6-Rx3(A7)

Use

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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DinoIII

Plasmid

#128100

Purpose

Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

RNA complex & upstream/downstream of LHC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Inserts are potential promoters

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIS2-TIP47 (Delta 1-190)

Plasmid

#17640

Depositor

James Hurley

Article

Hickenbottom et al Structure. 2004 Jul . 12(7):1199-207.

Insert

Tip47 (Delta 1-190) (Plin3 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Delta 1-190

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBiex-1 AC30_eGFP

Plasmid

#82715

Purpose

Expresses a GFP-tagged recombinant adenylyl cyclase.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)

Use

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSY6

Plasmid

#90482

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum

Depositor

Sheng Yang

Article

Shao et al Cell Res. 2007 Nov;17(11):963-5. doi: 10

Insert

group II intron and ItrA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKSS

Plasmid

#39272

Depositor

Peter Kast

Article

Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

Type

Empty backbone

Use

Positive-selection cloning vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pET15b-pA-Mnase

Plasmid

#124883

Purpose

To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

Depositor

Keji Zhao

Article

Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

Insert

protein A-MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBS mCD4

Plasmid

#14613

Depositor

Dan Littman

Article

Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

Insert

CD4 (Cd4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2007

Availability

Academic Institutions and Nonprofits only

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pET-PhoCl1-6His

Plasmid

#164033

Purpose

Expressed PhoCl1 in a pET expression vector

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl1

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template1-3XMyc-Spacer

Plasmid

#176208

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template1-3XMyc-Spacer

Use

Tags

Myc tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pZH521

Plasmid

#102674

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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