pKSS
(Plasmid #39272)

• Purpose: (Empty Backbone)
• Depositing Lab: Peter Kast
• Publication: Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

Backbone

• Vector backbone: pKSS
• Backbone manufacturer: Peter Kast
• Backbone size (bp): 4133
• Vector type: Bacterial Expression ; Positive-Selection Cloning Vector
• Selectable markers: PheS negative selection

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: This plasmid is provided in XL1 Blue. However, you can also use HB101 to propagate this plasmid if you prefer.
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pKSS Manual

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning vector for direct selection of insert-containing plasmids. Cloning into the plasmid and disrupting/removing the pheS gene enables plasmids containing insert to survive on agar plates containing p-chloro-phenylalanine (p-Cl-Phe) plus ampicillin.

Valuable for positive selection cloning. See Genbank accession number U01668 for more information.

Hennecke, H. & P. Kast. 1994/1996. "L-phenylalanyl-tRNA synthetase mutants, a process for the preparation thereof and the use thereof for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins" US patent no. 5,370,995 (requested Patent CA2059611 and AU625963) and US patent no. 5,536,652. Japanese patent no. 3102944. Patent applications pending in Canada.