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Plasmid#159527PurposeExpresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.DepositorArticleInsertCanavalia ensiformis pro-concanavalin A
UseTagsSix-Histidine tag and tobacco etch virus (TEV) pr…ExpressionBacterialMutationPromoterT5Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR284
Plasmid#125093PurposeExpression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143DepositorInsertsompR(REC)-manR(DBD)122
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMD14
Plasmid#138654PurposephiOZJ integrating Streptomyces expression vector, intact RDFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPermE* variant sequence from pDA1652Available sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPO685
Plasmid#195300PurposepBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.DepositorInsertMcCAST Cascade and native array with PaqCI entry site
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCT3-Halo
Plasmid#175322PurposeProduction of HaloTag fusion proteinDepositorInsertCCT3 (CCT3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
130_pSKB3-LUMP-P.leioghnati
Plasmid#65894PurposeBacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetimeDepositorInsertLUMP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBY3_ligD-HygR
Plasmid#104625PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.DepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationFnCpf1 codon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pRAV23
Plasmid#67715PurposeParental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseTagsExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP45-2A(sfGFP)
Plasmid#126989PurposeLevel 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_191
Plasmid#111821PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 171-191
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEA106
Plasmid#187872PurposeExpresses mCherry under the virB promoter.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAgrobacterium virB promoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-BioID2-HA
Plasmid#176744PurposeExpresses Ezrin fused with BioID2 at the C-terminus in astrocytesDepositorInsertEzr-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Spy0469
Plasmid#159996PurposeExpresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cellsDepositorInsertSpy0469 (24-389)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-Zif268
Plasmid#12610DepositorInsertZif268 binding site in pH3U3
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B MT
Plasmid#108855PurposeEncodes for the fluorescent protein mTurqoiseDepositorInsertmTurqoise
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV LIC cloning vector (2R-T)
Plasmid#29717DepositorTypeEmpty backboneUseTagsStrepII-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGST-PPARalpha
Plasmid#16550DepositorInsertPPAR alpha (PPARA Human)
UseTagsGSTExpressionBacterialMutationN-terminal DNA-binding domain: aa 1-249PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only