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PurposeParental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 3000
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Modifications to backboneInsertion between EcoRI and HindIII sites of MCS
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTranscription cassette for 3'-tagged RNAs
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SpeciesSynthetic
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Insert Size (bp)300
- Promoter T7 RNA polymerase
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRAV23 was a gift from Robert Batey & Jeffrey Kieft (Addgene plasmid # 67715 ; http://n2t.net/addgene:67715 ; RRID:Addgene_67715) -
For your References section:
Improved native affinity purification of RNA. Batey RT, Kieft JS. RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4. 10.1261/rna.528007 PubMed 17548432