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Plasmid#186271PurposeExpression vector of His-RanT24N in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis tagged and His-tagged at N terminalExpressionBacterialMutationChanged T24 into NPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAK462
Plasmid#140700PurposeNBαMs/Rt-IgG1-FcDepositorInsertNBαMs/Rt-IgG1-Fc
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCS Citrine
Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
t1-435
Plasmid#166578PurposeFor expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-435) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-435 onlyPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#48100PurposeCumate-inducible expression of ECFP-tagged fusion proteinDepositorTypeEmpty backboneUseTags3xFLAG and ECFPExpressionBacterialMutationPromoterPQ5Available sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSM3
Plasmid#49799PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
4LEC
Plasmid#60091PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21A:JMC072-B07:C227766 (METTL21A Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mem-mCherry-pTRIP
Plasmid#163521PurposeUsed to construct lentivirus to express membrane-targeted mCherry in mammalian cellsDepositorInsertmembrane targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK438
Plasmid#71590PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK240
Plasmid#71127PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
UseTagsGyrA intein and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcI-ts,ind+-(mtSSB delta20)
Plasmid#186280PurposeExpresses Human Mitochondrial SSB in E. coliDepositorInsertMitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
UseTagsExpressionBacterialMutationFirst 20 amino acids corresponding to probable mi…PromoterlambdaAvailable sinceJuly 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP48-2A(sfGFP)
Plasmid#126993PurposeLevel 2 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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DLG4_PDZ_3
Plasmid#103953PurposeProtein expression and purification of PSD95 PDZ3 domainDepositorInsertDLG4 (DLG4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-p47
Plasmid#21268DepositorInsertp47 (Nsfl1c Rat)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMLM36 (aka pBR-UV5-GP-FD2)
Plasmid#21871DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C3
Plasmid#50355PurposeMouse eIF2C3 cDNA cloneDepositorInserteIF2C3 (Ago3 Mouse)
UseTagsExpressionBacterialMutationPromoterT3Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase
Plasmid#196185PurposeCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only