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Plasmid#117393PurposeTn7 tagging vector with a LacI-controlled kill switch counterselection systemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS84, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKan cpRBP-FL P53
Plasmid#174504PurposeExpress Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)DepositorInsertP53 gene (TP53 Human)
UseTags20 amino acid linker containing HRV3C protease si…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCARSF63
Plasmid#64695PurposeH6-TRX-Smt3-GB1-NpuDnaEN102-CBDDepositorTypeEmpty backboneUseTagsCBD, HisTag, NpuDnaE-N102, Smt3, and TRXExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
BAT8 (3RJW)
Plasmid#28125PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBAT8 (EHMT2 Human)
UseTagsHis-tag with integrated thrombin siteExpressionBacterialMutationcontains amino acid residues 913-1193.PromoterAvailable sinceFeb. 10, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/HisB-Medium-FT
Plasmid#31917DepositorInsertMedium-FT
UseTagsHisExpressionBacterialMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJK217
Plasmid#72471PurposeProduces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK225
Plasmid#71124PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coliDepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
bfloGFPa1
Plasmid#60749PurposeBacterial expression of Amphioxus GFPa1DepositorInsertGFPa1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-FLAG-LEM
Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
UseTagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGL820
Plasmid#58221PurposeFor fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.DepositorInsertILV2
UseTagsExpressionBacterialMutationPromoterILV2 native promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1CCtoSS
Plasmid#89157PurposeRecombinant expression of PTBP1 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
UseTagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET28-HADH2
Plasmid#67863Purposebacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)DepositorInsertHADH2 (HSD17B10 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF57
Plasmid#17877DepositorInsertBAF57 (SMARCE1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed toehold switch
Plasmid#107353PurposeExpresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressedDepositorInserttoehold switch 1 + eforRed
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023
Plasmid#90317PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only