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Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
human p53-(82-360)
Plasmid#24868DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 82-360PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3.5VPIII.EGFP.IGR2.1
Plasmid#40865DepositorInsertMouse vasopressin gene (Avp Mouse)
UseTagsExpressionBacterialMutationPromoterAVPAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
UseTagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2 + sfGFP
Plasmid#88918PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS7k-RFP
Plasmid#35321DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_STRAW
Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_STRAW (mdh Streptomyces avermitilis)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsHis8ExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)
Plasmid#181788PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Z-UBQLN2-7
Plasmid#166563PurposescFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHJL2
Plasmid#199377PurposeEncodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6f::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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PclpB GFP pZa
Plasmid#170074PurposeGFP expression under the control of E. coli clpB promoterDepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-EFG1mt
Plasmid#31076DepositorInsertMature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
UseTagsHisExpressionBacterialMutationIncludes the coding sequence for the mature form.…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG/Q157TAG
Plasmid#68299PurposeE17/Q157 TAG GFP reporterDepositorInsertGFP E17TAG/Q157TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only