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Addgene
Showing: 6821 - 6840 of 27115 results

  2. pBTR(SMCytc)

    Plasmid
    #72330
    Purpose
    Expresses spider monkey cytochrome c along with the heme lyase (CYC3) from yeast for heme insertion
    Depositor
    Bruce Bowler
    Insert
    spider monkey cytochrome c gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  3. pJK683

    Plasmid
    #72378
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72378-plasmid-map-sequence-id-134010

    Enlarge

  4. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

    Enlarge

  5. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

    Enlarge

  6. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

    Enlarge

  7. pJK034

    Plasmid
    #72383
    Purpose
    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72383-plasmid-map-sequence-id-136200

    Enlarge

  8. pJK515

    Plasmid
    #72385
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylglycinamide formyltransferase 2
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72385-plasmid-map-sequence-id-133919

    Enlarge

  9. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

    Enlarge

  10. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

    Enlarge

  11. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

    Enlarge

  12. pJK630

    Plasmid
    #72389
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72389-plasmid-map-sequence-id-133978

    Enlarge

  13. pJK631

    Plasmid
    #72390
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    inserted Ala2 to facilitate cloning
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72390-plasmid-map-sequence-id-133987

    Enlarge

  14. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

    Enlarge

  15. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  16. pJK639

    Plasmid
    #72398
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72398-plasmid-map-sequence-id-133991

    Enlarge

  17. pJK649

    Plasmid
    #72399
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72399-plasmid-map-sequence-id-133993

    Enlarge

  18. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

    Enlarge

  19. pJK361

    Plasmid
    #72403
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72403-plasmid-map-sequence-id-134630

    Enlarge

  20. pJK365

    Plasmid
    #72404
    Purpose
    Produces Acetobacter aceti 1023 Est01
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioester hydrolase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72404-plasmid-map-sequence-id-134631

    Enlarge

Showing: 6821 - 6840 of 27115 results