-
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A53T (SNCA Human)
UseTagsExpressionBacterialMutationA53TPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
UseTagsExpressionBacterialMutationsplit before mutationsPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCherryExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-mcs
Plasmid#12609DepositorInsertMultiple cloning site in His3 Ura3 reporter plasmid
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-His
Plasmid#14030DepositorInsertCyPet
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (1M)
Plasmid#29656DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHis6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
UseTagsExpressionBacterialMutationH840A of Cas9PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GAPDH
Plasmid#83910Purposestable overexpressionDepositorInsertglyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YBX3-Halo
Plasmid#175308PurposeProduction of HaloTag fusion proteinDepositorInsertYBX3 (YBX3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
IF-GFP-ATRX
Plasmid#45444PurposeATRX expression vectorDepositorInsertATRX (ATRX Mouse, Human)
UseTagsGFP and HAExpressionBacterial and MammalianMutationisoform 2, missing codon E124PromoterPCMVAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWSK29
Plasmid#172972PurposeLow copy number cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
UseTagsGST solubility tag and HHHHHHExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDXinit
Plasmid#110101PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phagesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET YBX-1
Plasmid#13035DepositorInsertYBX1 (YBX1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only