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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD18-Cm
- Backbone size w/o insert (bp) 6026
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Vector typeBacterial Expression ; Yeast/Bacteria optimized codons
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAzurite
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (not destroyed)
- 3′ cloning site SfiI (not destroyed)
- 5′ sequencing primer See map (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB18cmBFPAzurite was a gift from Patrick Daugherty (Addgene plasmid # 14034 ; http://n2t.net/addgene:14034 ; RRID:Addgene_14034) -
For your References section:
Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Mena MA, Treynor TP, Mayo SL, Daugherty PS. Nat Biotechnol. 2006 Dec . 24(12):1569-71. 10.1038/nbt1264 PubMed 17115054