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Plasmid#89880PurposeStreptavidin that has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K121R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK121RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT204
Plasmid#134881PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D
Plasmid#159562PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)
UseTagsHis6-MBP-tev-AviExpressionBacterialMutationG12DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
NUPL2_ZNF_pGEX
Plasmid#135135PurposeRecombinant expression of NUPL2 with dual-affinity tagDepositorInsertNUPL2 (NUPL2 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G'
Plasmid#209124PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'DepositorInsertSUMO-A'-l4-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHYP-NPC-10H
Plasmid#162789PurposeSARS-CoV-2 Nucleocapsid Protein expression in E.coliDepositorInsertNucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
UseTags10xHisExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-null-GFPLVA
Plasmid#23338DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbPylRS(IPYE)
Plasmid#104072PurposeExpression plasmid encoding MbPylRS(IPYE) and pylTDepositorInsertPlpp MbPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmMaple3-CAM
Plasmid#101148PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple3
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pGT-Ah7
Plasmid#122574PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119067PurposeDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertNLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits