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pET11a_3xFlag-nsp14 (SARS-CoV-2)

Plasmid

#169159

Purpose

To express SARS-CoV-2 nsp14 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)

Use

Tags

3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(414)/GFP-AUT7(414)

Plasmid

#49424

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

Depositor

Daniel Klionsky

Article

Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
GST TopBP1 (aa 32-1522) His

Plasmid

#20375

Depositor

Aziz Sancar

Article

Choi et al Nucleic Acids Res. 2009 Jan 12. ():.

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Starts at aa32

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pET-45b-HOXD13-IDR-mCherry

Plasmid

#145280

Purpose

Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-IDR (HOXD13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23151

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgo

Plasmid

#210191

Purpose

Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Use

Tags

6x His tag and SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAN1201

Plasmid

#74695

Purpose

Circuit backbone plasmid (LacZa insert)

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJ23119-sgRNA

Plasmid

#113654

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter J23119 and sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-PUM1

Plasmid

#120384

Purpose

Bacterial expression of MBP-Pumillio1 fusion protein

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM1 (PUM1 Human)

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGBK CUP1 mGrape3

Plasmid

#170265

Purpose

Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3

Depositor

Bruce Hamilton

Article

Hernandez et al bioRxiv

Insert

momeric Grape 3

Use

Tags

GAL4 DNA Binding Domain and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

CUP1

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK2-1/1

Plasmid

#91377

Purpose

Protein expression and purification of human SH3 domain construct TNK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK2-1/1 (TNK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-MBP-4xDQNAT

Plasmid

#128390

Purpose

In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Maltose binding protein

Use

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

A302T- please see depositor comment

Promoter

T7

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDO23A

Plasmid

#27367

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

pUC/ori, bla

Use

Gateway donor vector

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pHS0663 pET45b(+) KraIscB-1 TwinStrep

Plasmid

#176536

Purpose

Bacterial expression of KraIscB-1-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

KraIscB-1

Use

Tags

TwinStrep

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIB185

Plasmid

#90196

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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MetNI (Lm)

Plasmid

#78316

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

Inserts

MetI
MetN

Use

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGGD_RT_li_Tq2CFP

Plasmid

#136971

Purpose

Goldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linker

Depositor

Klaus Theres

Insert

Tq2CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

cpGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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