-
Plasmid#118545PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
-
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX3x-hRanBP2-(IR1+M)
Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ2018
Plasmid#140665PurposedCas9, LuxR & Csy4DepositorInsertdCas9, LuxR & Csy4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Cdk9 T186A
Plasmid#28101DepositorInsertCdk9 (CDK9 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationContains a T186A mutation in Cdk9PromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorInsertHSPH1 (HSPH1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG2K
Plasmid#71742PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10
Plasmid#198449PurposeProduces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-Ad5RGDPK7_Δpvc10
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-His
Plasmid#119763PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6-spTorA-mCherry
Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
UseTags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-306
Plasmid#38315DepositorInsert5' 918 bp from STU2 ORF (STU2 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
UseTagsHis6 and StrepExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1007 (pSL6)
Plasmid#191002PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBA323
Plasmid#214751PurposeTo create delta(glmS,nadE) working strains and maintaining their viabilityDepositorInsertBBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
RAB3B (3DZ8)
Plasmid#32879PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB3B (RAB3B Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationamino acids 18-190 onlyPromoterAvailable sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only