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Plasmid#75006PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_27B_LacZ
UseTagsLacZExpressionBacterialMutationPromoterT7Available sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR PKAKTRn-Clover
Plasmid#177199PurposeGateway entry vector encoding PKAKTRnew-CloverDepositorInsertPKAKTRnew-mClover
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pDSG289
Plasmid#115608Purposesurface-displayed Nb2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pC011 LwCas13a locus with targeting spacer
Plasmid#91903PurposeLwCas13a locus into pACYC184 with targeting spacerDepositorInsertLwCas13a with targeting spacer
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS13: pTarget(AvCAST)
Plasmid#168146PurposeTarget plasmid containing AvPSP1 and attachement site for AvCAST.DepositorInsertsPAM for AvCAST + AvPSP1
glmS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorInsertG4G6 (GSN Human)
UseTags6xHis-MBPExpressionBacterialMutationWTPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIOM15
Plasmid#20869DepositorInsertaphA::aadA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-Nsp14-TEV-6His
Plasmid#154952PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp14-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbS2k-RFP
Plasmid#35330DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTetAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK642
Plasmid#72391PurposeProduces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsHis6ExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLibacceptorV2
Plasmid#106250PurposeRecombination-mediated cassette exchange with target genomic lociDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet-1_VNp-hGH
Plasmid#182411PurposeBacterial expression of Vesicle Nucleating peptide-human growth hormone fusionDepositorInsertVNp-hGH (GH1 Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-TolB-NHSTIIT-GG
Plasmid#215792PurposeCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEM10686
Plasmid#213995PurposePrpsM driven expression of mScarlet-IDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-LY-P2A
Plasmid#214895PurposeExpresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sitesDepositorInsertSPLICSFAST-LDh-LY-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only