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Plasmid#139755PurposeFor protein expression/crystallography (PDB:6F59)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6ExpressionBacterialMutationG177D; contains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN
Plasmid#200967Purposeinduction of phase-separated condensate mediated by FUSN-IDRDepositorInsertFUSN
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP)Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
KDM4D
Plasmid#73247PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKDM4D (KDM4D Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam02375:JmjN 18-64, pfam02373:JmjC 182-295PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKEW303-AcrVA5
Plasmid#115657PurposeExpresses AcrVA5 in bacteria, under TetR control.DepositorInsertAcrVA5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT003
Plasmid#182713PurposeaTc inducible dCasRx-IF3DepositorInsertdCasRx
UseCRISPRTags3x(GGGS)-Escherichia Coli Initiation Factor 3ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDH
Plasmid#133129PurposeA recombinant plasmid expressing mdh (malate dehydrogenase) geneDepositorInsertmalate dehydrogenase gene
UseTagsExpressionBacterialMutationPromotermalate dehydrogenase promoterAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1E10A
Plasmid#84607PurposePlasmid contains HCF-1rep1E10A with GST-tagDepositorInsertHCF-1rep1E10A (HCFC1 Human)
UseTagsGST and HisExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1wt
Plasmid#84606PurposePlasmid contains HCF-1rep1wt with GST-tagDepositorInsertHCF-1rep1wt (HCFC1 Human)
UseTagsGST-TEV and HisExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-galK
Plasmid#193727PurposeContains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galKDepositorInsertgalK (galK E. coli)
UseTagsExpressionBacterialMutationPromoterJ23113Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mNeptune2.5
Plasmid#104013PurposePlasmid encoding mNeptune2.5DepositorInsertmNeptune2.5
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (MSKGEE LIKENM… M11…PromoterproCAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ETV6-ETS
Plasmid#111180PurposeMinimal ETS domain of human ETV6 (TEL)DepositorInsertETV6 ETS domain (ETV6 Human, Mouse, Bovine)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU1098
Plasmid#14463DepositorInsertunstable gfp-mut3.1 LAA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCORE-UK
Plasmid#72238PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markersDepositorInsertsKlURA3
kanMX4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
UseTagsExpressionBacterialMutationPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-222PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -