pET28b-ETV6-ETS
(Plasmid #111180)

• Purpose: Minimal ETS domain of human ETV6 (TEL)
• Depositing Lab: Gregory Poon
• Publication: Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-13196. doi: 10.1074/jbc.M117.792887. Epub 2017 Jun 7.

Backbone

• Vector backbone: pET28b
• Backbone size w/o insert (bp): 5422
• Total vector size (bp): 5750
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ETV6 ETS domain
• Alt name: TEL
• Species: H. sapiens (human), M. musculus (mouse), B. taurus (bovine)
• Insert Size (bp): 300
• GenBank ID: XP_011518909
• Entrez Gene: ETV6 (a.k.a. TEL, TEL/ABL, THC5)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7promoter
• 3′ sequencing primer: T7terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gene encodes the DNA-binding domain of a human protein known as ETV6, whose gene we in turn obtained from the Harvard Plasmid Repository, so the sequence is already in the public domain. See https://www.ncbi.nlm.nih.gov/pubmed/28592487.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry