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Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP4657
Plasmid#107773PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pME6010
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB007(+)
Plasmid#99147PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
UseTagsExpressionBacterialMutationPromoterPT7 and PproKAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_mVenus
Plasmid#118861Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertmVenus
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest-557
Plasmid#161890PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coliDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c006
Plasmid#139755PurposeFor protein expression/crystallography (PDB:6F59)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6ExpressionBacterialMutationG177D; contains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23101-GFPuv
Plasmid#106388PurposepBBR1k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorInsertLC3A (MAP1LC3A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citrine2
Plasmid#110772PurposeYellow fluorescent protein with improved photostabilityDepositorInsertCitrine2
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7RNAP Cyslight V174C
Plasmid#205078PurposeT7RNAP protein expressionDepositorInsertT7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
UseTagsExpressionBacterialMutationC125S, C347S, C510S, C515S, C530S, C723S V174CPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
KDM4D
Plasmid#73247PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKDM4D (KDM4D Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam02375:JmjN 18-64, pfam02373:JmjC 182-295PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1E10A
Plasmid#84607PurposePlasmid contains HCF-1rep1E10A with GST-tagDepositorInsertHCF-1rep1E10A (HCFC1 Human)
UseTagsGST and HisExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1wt
Plasmid#84606PurposePlasmid contains HCF-1rep1wt with GST-tagDepositorInsertHCF-1rep1wt (HCFC1 Human)
UseTagsGST-TEV and HisExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKEW303-AcrVA5
Plasmid#115657PurposeExpresses AcrVA5 in bacteria, under TetR control.DepositorInsertAcrVA5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN
Plasmid#200967Purposeinduction of phase-separated condensate mediated by FUSN-IDRDepositorInsertFUSN
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3993 pET ubcH7
Plasmid#8654DepositorInsertUbcH7 (UBE2L3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-P3-sgRNAluc
Plasmid#85590PurposeIntegrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template forDepositorInsertsgRNA targeting firefly luciferase encoding gene
UseCRISPRTagsExpressionBacterialMutationPromoterP3Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only