-
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX
Plasmid#221377PurposeExpresses ATRX in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationchanged G736 to EPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
Plasmid#192273PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-mKate(KPS12)
Plasmid#133831PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPuppAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU1144
Plasmid#14471DepositorInsertmRFP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA2
Plasmid#211911PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 CC
Plasmid#24403DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains the CC domain (aa 151-242)PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCW17 - pAdapter
Plasmid#174664PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.DepositorInsertMS2 coat protein
UseTagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSM040
Plasmid#128294Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPdX
Plasmid#85084PurposeExpresses putidaredoxin in E. coliDepositorInsertPutidaredoxin
UseTagsHis6 tagExpressionBacterialMutationchanged Cysteine 73 to Serine and Cysteine 85 to …PromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-hKdm2b-CxxC-PHD
Plasmid#49216PurposeBacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.DepositorInsertKdm2b (KDM2B Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68
Plasmid#153527PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#68DepositorInsertSb#68
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pccGFPKAN
Plasmid#73649PurposeFluorescent reporter for transmembrane protein dimerization.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pSKDuet16
Plasmid#41684DepositorInsertNpuDnaE intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:ffluc
Plasmid#88900PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + firefly luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only