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Showing: 5841 - 5860 of 29412 results

  3. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  5. pDB038a

    Plasmid
    #99211
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 29 and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99211-plasmid-map-sequence-id-192359

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  8. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  14. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114069-plasmid-map-sequence-id-220520

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  17. ZFYVE16

    Plasmid
    #39099
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ZFYVE16 (ZFYVE16 Human)
    Use
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5841 - 5860 of 29412 results