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pJB166

Plasmid

#86998

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection

Depositor

Julien Berro

Article

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-147

Plasmid

#118563

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR2

Plasmid

#112813

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP24-1A(sfGFP)

Plasmid

#126971

Purpose

Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLBH533_MBP-Cas14b2 expression

Plasmid

#112506

Purpose

Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mCherry (KmR)

Plasmid

#177207

Purpose

Plasmid with p15A origin for constitutive mCherry expression in E. coli

Depositor

Alexey Merz

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Synthetic constitutive promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP5099

Plasmid

#128714

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopK1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5328

Plasmid

#128721

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopU1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5306

Plasmid

#128712

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopH1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5571

Plasmid

#128713

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopI1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5539

Plasmid

#128728

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAF1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5618

Plasmid

#128709

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopE1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5617

Plasmid

#128706

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopB1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV8-1

Plasmid

#123375

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-1 (TRAV8-1 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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4Z3C (TET3)

Plasmid

#119969

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

TET3 (TET3 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

contains amino acids 48-98

Promoter

T7

Available since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMRE155

Plasmid

#118505

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSNK

Plasmid

#135193

Purpose

Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

I-SceI lamba Red

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pBsVchCAST-pyrE

Plasmid

#203813

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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