pCPP5306
(Plasmid
#128712)
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PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/SD/D-TOPO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehopH1
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SpeciesPseudomonas syringae
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also reference: PMID 29742421.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCPP5306 was a gift from Alan Collmer (Addgene plasmid # 128712 ; http://n2t.net/addgene:128712 ; RRID:Addgene_128712) -
For your References section:
A survey of the Pseudomonas syringae pv. tomato DC3000 type III secretion system effector repertoire reveals several effectors that are deleterious when expressed in Saccharomyces cerevisiae. Munkvold KR, Martin ME, Bronstein PA, Collmer A. Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 10.1094/MPMI-21-4-0490. 10.1094/MPMI-21-4-0490 PubMed 18321194